In: OIE Terrestrial Manual; 2013. p. 1–14. The lesions of orf (contagious ecthyma) and sheep and goat pox differ in distribution to that of PPR, but animals recovering from PPR may develop proliferative growths on the lips resembling orf, and the virus may be involved in the pathogenesis of the condition. 2016;142:16–21. We listed five criteria by significance: 1) Mortality, 2); Clinical scoring; 3) Viral load in post mortem tissue; 4) Hyperthermia duration; 5) Viral load in swabs (number of positive and cycle threshold (Ct) values). Results of this study indicates that PPRV is an invasive infection in animals that in a short period, less than 10 days, invade all vital organs. In: Chapter 7.8, OIE Terrestrial Animal Health Code; 2016. p. 1–10. Goats are usually known to be more susceptible to the disease. volume 15, Article number: 452 (2019) PPR is widespread in Africa, Middle East and Southern Asia [3,4,5]. 2013;80:1–10. Animals were tested negative for PPRV specific antibody by ELISA using the blocking enzyme-linked immunosorbent assay (bELISA) and virus neutralization test (VNT). nasal and lacrimal discharges, coughing and dyspnea (Fig. Peste des petits ruminants (PPR) is a viral disease which primarily affects small ruminants, causing significant economic losses for the livestock industry in developing countries. PPR is an acute infectious viral disease of small ruminants, characterized by fever , oculo-nasal discharge, erosive stomatitis, conjunctivitis, gastroenteritis. J Gen Virol. Nasal and lacrimal secretion represent the first and the direct way of virus transmission. 2014;9:1–13. CAS  Munir M. Peste des Petits Ruminants Virus. Morbillivirus vaccines: recent successes and future hopes. Small Rumin Res. by Muhammad Munir, Siamak Zohari, Mikael Berg August 2012 Peste de Petits Ruminants (PPR) is a highly contagious viral disease of domestic and wild small ruminants that can significantly affect economies. In comparison with other strains from lineages II and IV, oral lesions were seen at 5–9 dpi, diarrhoea at 8–10 dpi and death 7–12 dpi [19]. The consent was obtained from MCI Santé Animale breeding farm to use the animals in the study. 1988;52:46–52. In the spleen, intestine, liver, kidney and heart virus charge Ct values were 30.3, 31.2, 32.6, 35.6 and 37.4 respectively. Article  The test was based on serial 1:3 dilutions of heat inactivated sera, mixed with a constant dose of PPR virus (100 TCID50), incubated for 1 h, and then inoculated onto Vero cells to be observed for neutralization of cytopathic effect (CPE) after 7 days of the incubation period at 37 °C with 5% of CO2. Evaluation of the virulence of some strains of peste-des-petits-ruminants virus (PPRV) in experimentally infected West African dwarf goats. One goat presented an important dyspnea, depressive comportment and alimentation decrease in the last days (Table 1). Jones BA, Rich KM, Mariner JC, Anderson J, Jeggo M, Thevasagayam S, et al. -. Goats of both groups showed a long hyperthermia period (above 39 °C) during 6 to 9 days. Infection chronology, virus circulation, and the disease early detection need to be better understood. doi: 10.1371/journal.pone.0087145., DOI: Early events following experimental infection with Peste-des-Petits ruminants virus suggest immune cell targeting. 2017;98:2635–2644. Hammouchi M, Loutfi C, Sebbar G, Touil N, Chaffai N, Batten C, et al. PPRV genome was not detected by PCR for all samples collected swabs and viremia of two goats of group III. Swabs were collected in 2 ml PBS supplemented and centrifuged at 2000 rpm for 20 min at 4 °C. Rectal temperatures were measured 3 days prior to experimental infection with PPRV (MOR15), and following infection every day until 9 dpi. 1). 1993;168:47–52. Kumar P, Tripathi BN, Sharma AK, Kumar R, Sreenivasa BP, Singh RP, et al. Briefly, 25 μl of sera samples were added to 96 and incubated for 1 h at 37 °C. All the samples were screened for viral genome detection by qRT-PCR. Although PPRV-infected animals were known to exhibit clinical signs similar to rinderpest [1], it was not until the late 1970’s that PPR pathogenesis was evaluated in … The isolation of peste des petits ruminants virus from northern India. Infection chronology, virus circulation, and the disease early detection need to be better understood. Virus isolation could be done on swabs or lung tissue. We selected a virulent strain recently isolated in Morocco and tested on susceptible Alpine goats which showed a high clinical score with mortality [24]. Peste des petits ruminants. Pathogenesis and Clinical signs: Pathogenesis of PPR virus is similar to that of rinderpest in cattle. Virus pathogenesis and genetics Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Age and species are the key factor to succeed a PPR experimental infection. Chowdhury EH, Bhuiyan AR, Rahman MM, Siddique MSA, Islam MR. Natural peste des petits ruminants virus infection in black Bengal goats: Virological, pathological and immunohistochemical investigation. Transmission occurs by direct or indirect contact with virus-contaminated excrements of … Kgotlele T, Macha ES, Kasanga CJ, Kusiluka LJ, Karimuribo ED, Van Doorsselaere J, Wensman JJ, Munir M, Misinzo G. Transbound Emerg Dis. Emikpe BO, Akpavie SO. Please enable it to take advantage of the complete set of features! Lung is the tissue with the highest viral detected charge (Ct 22.6 for Group II and 27.1 for Group I). Both goats of group I had a 1.5 log10 value of antibody neutralizing titer at 9 dpi by VNT. I. Pathogenesis in the normal and the immunized host. The neutralizing antibody titer was calculated in accordance with Reed and Muench method. All authors read and approved the final manuscript. For each individual goat, a severity score was calculated. KEYWORDS: Spectrochemical-Assay, PPR virus, MTT assay, Plaque assay, Trypan blue assay. 2012;160:240–4. PPR is stand for Pestis des pestitis ruminants. CAS  Experimental infection of sheep and goats with a recent isolate of peste des petits ruminants virus from Kurdistan. On live animals, early diagnostic may be easily done on lacrimal and rectal swabs. Hyperthermia duration criteria was calculated by number of day > 39 °C. Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection. in the digestive tract he intestinal mucosa of the small intestine were moderately congested. bELISA was developed, tested and validated by the Pan African Veterinary Vaccine Centre of the African Union (AU-PANVAC) [36].